SIL-SELECT PLUS™, FertiPro, Belgium

18.87₾ – 1,330.00₾

Sperm preparation media

Sil-Select Plus™ is our home made new, improved and ready-to-use gradient system for semen preparation. The following gradients are available:

  • Sil-Select Plus Upper layer (45%)
  • Sil-Select Plus 80%
  • Sil-Select Plus Lower layer (90%)
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Sil-Select Plus™ is our home made new, improved and ready-to-use gradient system for semen preparation. The following gradients are available:

  • Sil-Select Plus Upper layer (45%)
  • Sil-Select Plus 80%
  • Sil-Select Plus Lower layer (90%)

Sil-Select Plus™ gradients consist of silane-coated colloidal sicila particles suspended in HEPES-buffered Earle's balanced salts solution, and as from batch FP18SIP20 supplemented with 0.4 - 2.2g/l human serum albumin.

Extensive testing during the design of the product has shown it to be non-cytotoxic and non-irritant with a non-significant allergenic potential.

Sil-Select Plus™ is produced from raw colloidal silica, so we have full control over each step in the production process. We have developed a very accurate purification system which results in a very clean product with endotoxin levels below 0.5EU/ml.

The Sil-Select Plus™ density gradient system (45%-90%) consists of a Sil-Select Plus Upper Layer (45%) and a Sil-Select Plus Lower Layer (90%).

We also offer Sil-Select Plus in an 80% dilution for use in IVF procedures.

We recommend to watch our demonstration video of the density gradients:

https://www.youtube.com/watch?v=ehnyhdgpyig&feature=youtu.be

Sil-Select Plus™ complies with the following specifications:

pH 7.2 - 7.9 (7.2-7.6 at release)
Osmolality 280-310mOsm/kg (Upper Layer)
290-330mOsm/kg (Lower Layer)
290-330mOsm/kg (80%)
Sterility Sterile
Endotoxine < 0.5 EU/mL
Sperm Survival test ≥ 80% survival after 4 hours exposure of density selected spermatozoa to the test medium; and ≥ 75% survival after 24 hours exposure of density selected spermatozoa to the test medium
Density 1.1050-1.1150g/ml (Lower Layer)
1.097-1.107g/ml (80%)
Viscosity in cP (25°C) <1.65cP (Lower Layer, 80%)
Albumin FDA (USA) and EMEA (Europe) compliant
Shelflife 18 months from date of produce

Regulatory

Europe: CE-marked - Canada: Health Canada License - Brazil: registered

Product order codes:

SIP008 Sil-Select Plus - 8 preparations
SIP016 Sil-Select-Plus - 16 preparations
SIP050 Sil-Select Plus - 2 x 50 ml
SIP100 Sil-Select Plus - 2 x 100 ml
SIP80_100 Sil-Select Plus 80% - 100 ml

The product composition can be found in the MSDS (see Resources). Additional information on some components is provided below:

Component Benefit
HEPES (18 mM) HEPES stabilizes the pH under air (Clark and Swain 2014), therefore CO2 incubation is not required.
Earle’s Balanced Salt Solution (EBSS) The composition of the salt solution results in an optimum pH and osmolality environment for sperm cells.
Silane coated colloidal silica Density gradient media have been optimized for maximum efficiency in the selection of spermatozoa for use in ART. Their density is directly linked with their functionality and depends on the % silanized colloidal silica particles added in the media.
HSA (0.4 - 2.2 g/L) Sil-Select Plus contains 0.4 - 2.2 g/Liter Human Serum Albumin (HSA) to optimize medium performances. HSA is universally added to most ART media because it is widely considered to be of benefit.
The role of albumin in ART media is extensive, including:
- Stabilization of the cell membrane of the embryo in the medium (Malda, et al. 2008).
- Inhibition of lipid peroxidation that can be damaging to sperm (Alvarez en Storey 1995).
- Carrier and source of essential molecules needed by the embryo (Malda, et al. 2008)
- Detoxification by binding waste products from cell metabolism (Blake, et al. 2004)
- Facilitating gamete/embryo manipulation by preventing adsorption to the surface through saturation of potential binding sites (Blake, et al. 2004)

Manufacturer: Beernem, Belgium

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